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KMID : 1023620060300010065
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.65 ~ p.70
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Apoptosis and Development of Porcine Parthenogenetic Embryos Activated and Cultured in Different Condition
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Hwang In-Sun
Seo Jin-Sung
Cheong Hee-Tae
Im Gi-Sun
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Abstract
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This study investigated apoptosis and in vitro development of parthenogenetic preimplantation porcine embryos. In vitro matured oocytes for were used. Apoptotic cell death was analyzed by using a terminal deoxynucleatidyl transferase mediated deoxyuridine 5-triphosphate nick-end tabling (TUNEL) assay. In experiment 1, oocytes were activated with two electric pulses (CH) of 1.2 kV/cm for (E), E + 6-dimethylaminopurine (6-DMAP) or E + cycloheximide (CH) and cultured in PZM-3 under 5% in air at . In experiment 2, oocytes were activated by E and cultured in PZM-3 or NCSU-23 under a gas atmosphere of 20% (, in air) or 5% . Oocytes activated with E+6-DMAP or E+CH showed higher blastocyst rates (36.3% and 32.5%) compared to E alone (27.7%). The frequency of apoptosis according to treatments were 5.3%, 7.7% and 7.1% respectively. Oocytes activated with E alone showed lower (P<0.05) frequency of apoptosis compared to other groups. In experiment 2, parthenotes cultured in PZM-3 showed slightly higher blastocyte rates (28.2% and 29.7%) compared to NCSU-23 (22.6% and 24.4%) regardless of atmosphere. Blastocysts generated in PZM-3 showed lower (P<0.05) apoptosis rate under 20% (9.2% vs 16.9%), whereas those in NCSU-23 had slightly lower apoptosis rate under 5% (14.0% vs 18.4%). This result represents that activation method and culture condition could affect the frequency of apoptosis as well as in vitro developmental rate.
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KEYWORD
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Apoptosis, Parthenogenesis, Activation, In vitro culture
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