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KMID : 1023620060300030207
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Reproductive and Developmental Biology
2006 Volume.30 No. 3 p.207 ~ p.212
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Effect of Mechanical Enucleation Methods on Development of Bovine Nuclear Transfer Embryos
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Kim Se-Woong
Yang B.-S.
Cheong Hee-Tae
Seong Hwan-Hoo
Yang Byoung-Chul
Hwang In-Sun
Kim Dong-Hoon
Im Gi-Sun
Lee Min-Jung
Bae Sung-Hoon
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Abstract
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The aim of this study was to examine the effect of mechanical enucleation methods, aspiration and squeezing, on the developmental ability of nuclear transfer bovine embryos. Enucleated oocytes made by both enucleation methods were fused to adult ear skin cells. After 7 days of culture, developmental ability up to blastocyst stage was similar in both squeezing() and aspiration enucleation methods(). The proportion of blastocysts at Day 8 of culture was also similar between the aspiration() and squeezing enucleatign s(). The mean cell number in Day 7 blastocysts was also similar between the both groups(aspiration: vs. squeezing: ). The ratio of apoptotic cells was also found to be not significant different between the both groups(aspiration: vs. squeezing: ). These results suggest that aspiration and squeezing methods, as mechanical enucleation technique, are both useful for the production of bovine somatic cell nuclear transfer embryos.
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KEYWORD
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Nuclear transfer, Mechanical enucleation, Aspiration, Squeezing, Bovine
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