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KMID : 1023620070310020077
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Reproductive and Developmental Biology
2007 Volume.31 No. 2 p.77 ~ p.82
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Effects of Oocyte Maturational Age and Activation Conditions on the Development of Porcine Parthenogenetic Embryos
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Kwon Dae-Jin
Park Joo-Hee
Park Choon-Keun
Yang Boo-Keun
Cheong Hee-Tae
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Abstract
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This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for ; ES), 2) double electric stimulations (1.5 kV/cm for , followed by 1.0 kV/cm for after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6-7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.
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KEYWORD
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Parthenogenesis, Maturational age, Activation condition, cdc2 kinase activity, Porcine oocytes
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