|
KMID : 1023620070310020109
|
|
Reproductive and Developmental Biology
2007 Volume.31 No. 2 p.109 ~ p.113
|
|
|
Effect of Cytochalasin B in Activation Medium on the Development of Rat Somatic Cell Nuclear Transfer Embryos
|
|
Roh Sang-Ho
|
|
|
Abstract
|
|
|
|
This study was conducted to evaluate the effect of cytochalasin B (CB) treatment in the activation medium on the development of somatic cell nuclear transfer (SCNT) rat embryos. Fetal fibroblast cells were isolated from a Day 14.5 fetus, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM with or without CB for 4 hr, and formation of pseudo-pronucleus (PPN) was checked at 18 hr after activation. Then, they were transferred into day 1 pseudopregnant recipients (Hooded Wistar) or cultured for 5 days to check their developmental competence in vivo or in vitro. The number of PPN was not affected by CB treatment during the activation. However, CB treatment supported pre-implantation development of rat SCNT embryos. Embryos generated by the procedures of SCNT were also capable of implanting, with 1 implantation scar found from a recipient following the transfer of 87 SCNT embryos to four foster mothers. The result of the present study shows that rat SCNT embryo can develop to post-implantation stage following treatment with CB.
|
|
|
KEYWORD
|
|
|
Somatic cell nuclear transfer, Oocyte activation, Implantation, Cytochalasin B treatment, Rat
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|