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KMID : 1023620090330040203
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Reproductive and Developmental Biology
2009 Volume.33 No. 4 p.203 ~ p.209
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Relationship between Plasminogen Activity and Plasminogen Inhibitor during the Culture of Porcine Oviduct Epithelial Cells
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Ahn Shin-Hye
Cheong Hee-Tae
Yang Boo-Keun
Kim Dae-Young
Park Choon-Keun
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Abstract
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The present study was performed to identify changes of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) in porcine oviduct epithelial cells (POECs) during the estrous cycle. POECs obtained from ovary in pre?ovulatory (Pre?Ov), early to mid?luteal stage (Early?mid L) and post?ovulatory stage (Post?Ov). For the examine of PA activity, 1×105 fresh cells of POECs were cultured in DMEM/Ham F?12 containing 10% FBS and 0.2% amphotericin under humidified atmosphere of 5% CO2 in air and 38℃. The urokinase?type PA (uPA) was observed at 7 days of POECs culture. PA activity was measured with culture prolonged of 0, 3, 6, 12 and 24 h after culture of 7 days. The PA activity were high significantly (p<0.05) at 12 h of culture, but PA activity were decreased with culture periods increased. The PA activity in POECs of Post?Ov stage were higher significantly (p<0.05) than that of Early?mid L and Pre?Ov stage. When PAI?1 and PAI?2 were added during the POECs culture, the PA were observed significant low activity (p<0.05). The PA activity and protein expression were decreased by PA inhibitor. This results suggest that PAI?1 and PAI?2 have a suppressive action on change of PA activity during the estrous cycle of pigs. Specifically, this study using PA inhibitor was effect the PA activity and PAI expression in oviduct epithelial cells in pigs.
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KEYWORD
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Plasminogen activator, Oviduct epithelial cells, Estrous cycle, PA-inhibitor, Pig
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