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KMID : 1023620100340030161
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.161 ~ p.167
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Development of Heterologous ELISA System for Diagnosis of Pregnancy in Swine
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Park Sung-Min
Lee An-Na
Yoon Taek-Joon
Park Yong-Soo
Song Tae-Jun
Kim Young-Hoon
An Hyo-Sun
Park Won-Choul
Kim Jong-Bae
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Abstract
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Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with E1S. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50～9.67% and 8.50～9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29～30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.
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KEYWORD
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Oestrone sulfate, Oestrone-3-glucuronide, Pregnancy, Antigen, Heterologous, Competitive ELISA
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