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KMID : 1023620100340030175
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.175 ~ p.180
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Efficient Labeling of Porcine Hematopoietic Cells by Fluorescence-Conjugated Nanoparticles
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Lee Hyun-Joo
Park Eun-Ji
Lee Yong-Soo
Park Sung-Won
Kim Jae-Hwan
Kim Dong-Ku
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Abstract
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Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.
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KEYWORD
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Silica-coated nanoparticles, Porcine hematopoietic cells, RITC, Biocompatibility, Confocal microscopy
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