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KMID : 1023620100340030235
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.235 ~ p.240
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Proteomic Analysis of Differentially Expressed Plasma Proteins during Early Pregnancy in Hanwoo
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Kim Pyung-Hee
Kwon Dae-Jin
Oh Keon-Bong
Lee Hwi-Cheul
Yang Byoung-Chul
Im Gi-Sun
Min Kwan-Sik
Yoon Jong-Taek
Jin Dong-Il
Hwnag Seong-Soo
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Abstract
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This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo
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KEYWORD
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Plasma protein, Early pregnancy, 2DE, Haptoglobin, Paraoxonase
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