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KMID : 1023620100340030257
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.257 ~ p.262
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Retrovirus Vector-Mediated Gene Transfer to the Chicken Blastodermal Cells Cultured In Vitro
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Park Sung-Joon
Koo Bon-Chul
Kwon Mo-Seon
Kim Teo-An
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Abstract
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The purpose of this study is to establish a basic culture system enabling in vitro culture of chicken blastodermal cells and to test the feasibility of retrovirus-mediated gene transfer to the cultured cells. The blastodermal cells were isolated from freshly laid eggs of stage X and cultured with or without STO feeder layer cells. Stem cell-like morphology was maintained after multiple passages and RT-PCR analysis proved expression of several stem cell specific genes. Immunocytochemical analysis using antibodies of anti-EMA-1 and anti-SSEA-1 also showed the feature of stem cells. Infection of the cultured blastodermal cells with LNCGW retrovirus vector resulted in successful transfer of foreign genes. The results of this study may be useful in establishing stem cell-mediated transgenic chicken production.
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KEYWORD
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Chicken blastodermal cell, RT-PCR, Immunocytochemical analysis, Retrovirus vector, Transgenic chicken
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