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KMID : 1023620100340030264
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Reproductive and Developmental Biology
2010 Volume.34 No. 3 p.264 ~ p.270
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Protein Patterns on a Corpus Luteum during Pregnancy in Korean Native Cows
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Chung Hak-Jae
You Dong-Min
Kim Hyo-Ju
Choi Hye-Young
Lee Myeong-Suk
Kim Jin-Bum
Lee Suck-Dong
Park Jung-Yong
Lee Myeung-Sik
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Abstract
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Luteal cells produce progesterone that supports pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. In the present study, the corpus luteum (CL) in early pregnancy established from luteal phase and pregnant phase was analyzed. The first study determined progesterone changes in the bovine CL at day 19 (early maternal recognition period) and day 90 in mid-pregnancy and compared them to the CL from day 12 of the estrous cycle. CL alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing CL from luteal phase to those from pregnant phase counterparts, significant changes in expression level were found in 23 proteins. Of these proteins 17 were not expressed in pregnant phase CL but expressed in luteal phase counterpart, whereas, the expression of the other 6 proteins was limited only in pregnant phase CL. Among these proteins, vimentin is considered to be involved in regulation of post-implantation development. In particular, vimentin may be used as marker for CL development during pregnancy because the expression level changed considerably in pregnant phase CL tissue compared with its luteal phase counterpart. Data from 2-DE suggest that protein expression was disorientated in mid pregnancy from luteal phase, but these changes was regulated with progression of pregnancy. These findings demonstrate CL development during mid-pregnancy from luteal phase and suggest that alternations of specific CL protein expression may be involved in maintenance of pregnancy.
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KEYWORD
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Hanwoo, Luteal and pregnancy phase, 2-DE, Protein expression patterns
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