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KMID : 1023620110350030295
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Reproductive and Developmental Biology
2011 Volume.35 No. 3 p.295 ~ p.300
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Effect of Methyl-Beta-Cyclodextrin (MBCD) on Cryopreservation of Bull Spermatozo
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Lee Kyung-Jin
Seo Gi-Beom
Lee Yong-Seung
Yoo Han-Jun
Cheong Hee-Tae
Lee Seung-Hwan
Yang Boo-Keun
Park Choon-Keun
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Abstract
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The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.
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KEYWORD
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Methyl-beta-cyclodextrin, Cholesterol, Cryopreservation, Flow-cytometry, Bull sperm viability
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