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KMID : 1023620110350030301
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Reproductive and Developmental Biology
2011 Volume.35 No. 3 p.301 ~ p.306
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Growth Factors Supplementation in Culture Medium Leads to Active Proliferation of Porcine Fibroblasts
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Kim Bella
Ko Na-Young
Hwang Seong-Soo
Im Gi-Sun
Kim Dong-Hoon
Park Jin-Ki
Ryoo Zae-Young
Oh Keon-Bong
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Abstract
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Fibroblasts of large animals are easy to isolate and to maintain in vitro culture. Thus, these cells are extensively applied to donor cell for somatic cell nuclear transfer, and to substrate cells to generate induced pluripotent stem cells after transfection of requited genes to be essentially required for direct reprogramming. However, limited mitotic activity of fibroblasts to differentiate along a terminal lineage becomes restrictive for their versatile application. Recently, commercial culture medium and systems developed for primary cells are provided by manufactures. In this study, we examined whether one of the systems developed for primary fibroblasts of human are effective on porcine ear skin fibroblasts. To this end, we performed proliferation assay after five days culture in vitro of porcine fibroblasts in medium DMEM, which is generally used for fibroblasts culture, and medium M106 for human dermal fibroblasts, supplemented with various concentrations of FBS and LSGS contained mainly growth factors, respectively. Consequence was that presence of 15% FBS and 0.1 ${\times}$ 수식 이미지 concentrations of LSGS in DMEM showed most active proliferation of porcine fibroblasts.
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KEYWORD
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Fibroblasts, Culture medium, Proliferation
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