KMID : 1023620110350030313
|
|
Reproductive and Developmental Biology 2011 Volume.35 No. 3 p.313 ~ p.317
|
|
The Effect of Oocyte Donor Age and Micromainpulation Medium on the Development of Mouse Cloned Embryos
|
|
Kim Dong-Hoon
Lee Youn-Su Oh Keon-Bong Hwnag Seong-Soo Im Gi-Sun Park Jin-Ki
|
|
Abstract
|
|
|
This study was conducted to examine the effect of oocyte donor age and micromanipulation medium on the development of mouse cloned embryos receiving cumulus cells. Mouse oocytes were obtained from 6 to 11 week-old mice BDF1 female mice(experiment 1) and cumulus cells were used as donor cells. Micromanipulation procedures for nuclear transfer(NT) were performed in FHM, M2 or Hepes-buffered TCM199(TCM199) medium(experiment 2). After nuclear transfer, the reconstructed oocytes were activated by 10 mM SrCl2 in Ca-free CZB medium in the presence of 5 II ¥ìg/ml cytochalasin B for 5 h and cultured in KSOM medium for 4 days. In experiment 1, the survival rate of oocytes after injection of cumulus cells were significantly(p<0.05) lower in oocytes from 6~7 week-old mice(53.3%) than in oocytes from 8~9(80.9%) and 10~11 week-old mice(77.1%). In experiment 2, the survival rate of oocytes after cell injection were significantly(p<0.05) higher in FHM and M2 medium(71.7% and 76.9%) than in TCM199 medium(51.2%). The activation rates of cloned embryos were not different among the micromanipulation media. However, the embryos developed to blastocyst stage were significantly(p<0.05) higher in FHM medium(13.9%) than in M2 and TCM199 medium(0.0% and 0.0%). In conclusion, the present study suggest that oocytes from above 8 week-old mice are superior to oocytes from 6~7 week-old mice as a source of recipient cytoplasm and FHM is superior to M2 and TCM199 as a micromanipulation medium for mouse somatic cell cloning.
|
|
KEYWORD
|
|
Mouse cloning, Micromanipulation medium, Oocyte donor age
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|