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KMID : 1023620110350030363
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Reproductive and Developmental Biology
2011 Volume.35 No. 3 p.363 ~ p.367
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Effect of Magnetized Water on Cryopreservation in Bovine Spermatozoa
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Seo Gi-Beom
Lee Yong-Seung
Lee Kyung-Jin
Yoo Han-Jun
Cheong Hee-Tae
Yang Boo-Keun
Lee Seung-Hwan
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Abstract
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The purpose of this study was to improve of frozen-thawed sperm using magnetized water in Korean native cattle.
Before cryopreservation, without egg-yolk Triladyl ® solution was flowed though magnet [0, 2000, 4000 and 6000 Gauss(G)] for 5 min. The freezing of dilluted semen added with Triladyl containing 20% egg-yolk. Analysis of frozen- hawed sperm was estimated viability with SYBR14/PI double stain, membrane intact with hypoosmotic swelling test (HOST), acrosome reaction with FITC-PNA, mitochondria membrane function with Rhodamin 123 by flow- cytometry. Sperm viability was significantly higher in 4000G group than other groups (p<0.05). However, the Hypoosmotic Swelling Test(HOST) was significantly higher in fresh, 4000 and 6000G than 0 and 2000G (p<0.05). In addition, mitochondria membrane damage and acrosome damage were significantly lower in 6000G group than other groups (p<0.05). in conclusion we suggest that magnetized water could be improve ability of sperm on cryopreservation in Korean native cattle.
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KEYWORD
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Korean native cattle, Spermatozoa, Cryopreservation, Magnetized water
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