|
KMID : 1023620110350030369
|
|
Reproductive and Developmental Biology
2011 Volume.35 No. 3 p.369 ~ p.375
|
|
|
Effect of α-Tocopherol Treatment and Freezing for In Vitro Bovine Embryo Production in Korean Native Cows
|
|
Jung Jin-Woo
Son Jun-Kyu
Choe Chang-Yong
Suh Sang-Won
Choi Jin-Seok
Cho In-Cheol
Han Sang-Hyun
Kang Tae-Young
Kang Min-Soo
Kim Deog-Im
Kim Hyun-Jong
Cho Sang-Rae
|
|
|
Abstract
|
|
|
The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival
of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.
|
|
|
KEYWORD
|
|
|
Bovine, Embryos, In vitro, In vivo, Vitrification
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|