|
KMID : 1023620110350040503
|
|
Reproductive and Developmental Biology
2011 Volume.35 No. 4 p.503 ~ p.510
|
|
|
Toxicity and Biomedical Imaging of Fluorescence-Conjugated Nanoparticles in Hematopoietic Progenitor Cells
|
|
Min Gye-Sik
Kim Dong-Ku
|
|
|
Abstract
|
|
|
Cellular uptake of nanoparticles for stem cell labeling and tracking is a critical technique for biomedical therapeutic
applications. However, current techniques suffer from low intracellular labeling efficiency and cytotoxic effects, which
has led to great interest in the development of a new labeling strategy. Using silica-coated nanoparticles conjugated
with rhodamine B isothiocyanate (RITC) (SR), we tested the cellular uptake efficiency, biocompatibility, proliferation
or differentiation ability with murine bone marrow derived hematopoietic stem/progenitor cells. The bone marrow hematopoietic cells showed efficient uptake with SR with dose or time dependent manner and also provided a higher
uptake on hematopoietic stem/progenitor cells. Biocompatibility tests revealed that the SR had no deleterious effects
on cell cytotoxicity, proliferation, or multi-differentiation capacities in vitro and in vivo. SR nanoparticles are advantageous
over traditional labeling techniques as they possess a high level of cellular internalization without limiting
the biofunctionality of the cells. Therefore, SR provides a useful alternative for gene or drug delivery into hematopoietic
stem/progenitor cells for basic research and clinical applications
|
|
|
KEYWORD
|
|
|
cHematopoietic stem/progenitor cells, Silica nanoparticles, Biocompatibility, In vivo transplantation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|