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KMID : 1023620120360020109
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Reproductive and Developmental Biology
2012 Volume.36 No. 2 p.109 ~ p.114
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Polyclonal Antibody to a 37-kDa Recombinant Protein Derived from Bovine 20α-Hydroxysteroid Dehydrogenase
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Naidansuren Purevjargal
Min Kwan-Sik
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Abstract
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We prepared the polyclonal antibody anti-20α-hydroxysteroid dehydrogenase (anti-20α-HSD) against the recombinant full-length protein bovine 20α-HSD in Escherichia coli. The specificity of anti-20α-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine 20α-HSD and bovine placental tissues. According to western blot analysis, anti-20α-HSD specifically recognizes the 37-kDa protein bovine 20α-HSD. The protein is not present in untransfected CHO cells. Anti-20α-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine 20α-HSD protein in the cultured luteal cells during the estrous cycle later.
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KEYWORD
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Polyclonal antibody, Bovine, 20α-HSD, Ovary, Placenta
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