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KMID : 1023620130370010017
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Reproductive and Developmental Biology
2013 Volume.37 No. 1 p.17 ~ p.22
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Identification of Stage-specific Genes Related to Porcine Folliculogenesis
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Lee Jae-Hee
Lee Seung-Tae
Kim Hee-Bal
Lim Jeong-Mook
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Abstract
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Although assisted reproductive technology is very useful to develop novel and therapeutic biomaterials for reproduction, research on molecular mechanism of folliculogenesis in pig is not clear. Therefore, the alteration of gene expression during follicular development in pigs was examined in this study. The expression of folliculogenesis-related genes was quantified in preantral (250~300㎛) and antral (> 300㎛ in diameter) follicles, and overall gene expression was evaluated by a genome-wide microarray. The microarray results showed that 219 genes were differentially expressed, and of those, 10 and 22 known genes showed higher and less expression at the preantral stage than at antral stages, respectively. Among them, the expression of NR0B1, PPARG, GATA4, and ANXA2 genes related to folliculogenesis was validated by quantitative real-time PCR analysis. The expression of PPARG and GATA4 genes were increased at antral stages, but a significantly stage-specific increase (p<0.05) was only detected in annexin A2 (ANXA2) in antral-stage follicles. The expression of NR0B1 genes was increased at preantral stage and these patterns of gene expression were comparable to the results obtained by microarray analysis. We propose that the systematical regulation of genes supporting specific follicle stage should be employed for improved in-vitro folliculognesis.
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KEYWORD
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Antral follicles, Gene expression, Ovary, Preantral follicles
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