|
KMID : 1023620130370040233
|
|
Reproductive and Developmental Biology
2013 Volume.37 No. 4 p.233 ~ p.245
|
|
|
Transcriptional Profiling of Differentially Expressed Genes in Porcine Satellite Cell
|
|
Jeong Jin-Young
Kim Jang-Mi
Rajesh Ramanna Valmiki
Suresh Sekar
Jang Gul-Won
Lee Kyung-Tai
Kim Tae-Hun
Park Mi-Na
Cho Yong-Min
Lee Hyun-Jeong
Jeong Hak-Jae
Kim Kyung-Woon
|
|
|
Abstract
|
|
|
|
Muscle satellite cell (SC) is responsible for postnatal muscle growth, repair, and regeneration. Satellite cell is an important source of multi-potent stem cell process and differentiation into adipogenic, myogenic, and osteoblastogenic. The objective of this study was to identify alter of transcriptome during differentiation in porcine satellite cell and to elevated transcriptome at different stages of postnatal development to gain insight into the differences in differentiated PSC. We used RNA-seq technique to investigate the transcriptomes during differentiation in pig muscle. Sequence reads were obtained from Illumina HiSeq2000. Differentially expressed genes (DEG) were detected by EdgeR. Gene ontology (GO) terms are powerful tool for unification among representation genes or products. In study of GO biological terms, functional annotation clustering involved in cell cycle, apoptosis, extracellular matrix, phosphorylation, proteolysis, and cell signaling in differences stage. Taken together, these results would be contributed to a better understanding of muscle biology and processes underlying differentiation. Our results suggest that the source of DEGs could be better understanding of the mechanism of muscle differentiation and transdifferentiation.
|
|
|
KEYWORD
|
|
|
Microarray, Postnatal, Satellite cell, Muscle, Gene ontology
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|