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KMID : 1023620130370040247
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Reproductive and Developmental Biology
2013 Volume.37 No. 4 p.247 ~ p.253
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Comparison of Plasma Proteome Expression between the Young and Mature Adult Pigs
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Jeong Jin-Young
Nam Jin-Sun
Kim Jang-Mi
Jeong Hak-Jae
Kim Kyung-Woon
Lee Hyun-Jeong
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Abstract
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Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.
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KEYWORD
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Proteome, Porcine, Mature, MALDI-TOF-TOF, LC-MS-MS
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