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KMID : 1023620140380010053
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Reproductive and Developmental Biology
2014 Volume.38 No. 1 p.53 ~ p.62
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Relative Analysis between Fertility and Protein Changes in Semen of Different Species in Pigs
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Lee Yeon-Ju
Lee Sang-Hee
Kim Yu-Jin
Cheong Hee-Tae
Yang Boo-Keun
Park Choon-Keun
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Abstract
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The objective of this study was to investigate the relationship between fertility and protein pattern change using in vitro fertilization, analysis of sperm characteristics and two-dimensional gel electrophoresis in different pig types. In results, the viability and mitochondria integrity of sperm were higher significantly (p<0.05) but the portions of acrosome reaction was lower significantly (p<0.05) in Duroc and F₁ (potbellied × PWG miniature pig) than PWG miniature. On in vitro fertilization to investigate fertility, the fertility of F₁ semen war higher significantly (p<0.05) than in Duroc and PWG miniature pig. On the other hand, protein patterns showed similar function among the different boar semen. Especially, the heat shock 70 kDa 1-like and G patch domain-containing protein 4 were significantly (p<0.05) higher expressed in F₁ than in Duroc and PWG miniature pig. The proteins associated with mitochondria in Duroc were significantly (p<0.05) higher expressed than in F₁ and PWG miniature pig. The developmental rates to blastocyst stage of oocytes fertilized with sperm of F₁ pig were significantly (p<0.05) higher than in PWG miniature pig. However, phosphoglycerate kinase 2 and zinc finger protein 431 were significantly (p<0.05) higher expressed in PWG miniature pig than in F₁ and Duroc pigs. In conclusion, the results of the present study indicate that different proteins were expressed in different pig types, and were associated with a sperm functions and embryo development.
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KEYWORD
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Boar sperm, Protein patterns, In vitro fertility, Embryo development, Sperm characteristics
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