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KMID : 1023620140380020085
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Reproductive and Developmental Biology
2014 Volume.38 No. 2 p.85 ~ p.91
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Effects of Suspension Culture on Proliferation and Undifferentiation of Spermatogonial Stem Cells Derived from Porcine Neonatal Testis
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Park Min-Hee
Park Ji-Eun
Kim Min-Seong
Lee Kwon-Young
Yun Jung-Im
Choi Jung-Hoon
Lee Eun-Song
Lee Seung-Tae
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Abstract
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Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cultured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non-proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn’t stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.
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KEYWORD
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Pig, Suspension culture, Spermatogonial stem cells, Proliferation, Undifferentiation
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