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KMID : 1023620140380040143
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Reproductive and Developmental Biology
2014 Volume.38 No. 4 p.143 ~ p.146
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3-Hyroxyflavone in Maturation Medium Supports In Vitro Development of Fertilized Bovine Follicular Oocytes
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Kim Se-Woong
Park Jong-Im
Jung Yeon-Gil
Roh Sang-Ho
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Abstract
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Antioxidants, as reactive oxygen species scavengers, are one of the beneficial additives in serum-free defined culture medium. In this study, three separate experiments were performed to determine the effects of 3-hyroxyflavone added to the culture medium on the developmental competence of follicular bovine oocytes during in vitro maturation (IVM) and/or in vitro culture (IVC). The rate of blastocyst developed from oocytes cultured in IVM medium with 3-hyroxyflavone was significantly higher than that from control oocytes (39.0% vs. 26.3%, p<0.001), respectively. However, oocytes cultured in the medium with addition of 3-hyroxyflavone only at IVC period did not show significance in the blastocyst development when compared with control. When 3-hyroxyflavone was added to both IVM and IVC media, the rate of blastocyst formation was even significantly lower (21.1%) than control (26.5%; p<0.05). The present findings suggested that antioxidative activity of 3-hydroxyflavone added to only IVM medium beneficially affected the developmental competence of follicular bovine.
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KEYWORD
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3-Hyroxyflavone, In vitro maturation, In vitro culture, Bovine, Oocyte, Embyo
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