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KMID : 1023620160400010001
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Reproductive and Developmental Biology
2016 Volume.40 No. 1 p.1 ~ p.5
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Effects of L-Carnitine and Nicotinic Acid on Sperm Characteristics in Miniature Pigs
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Lee Yeon-Ju
Lee Sang-Hee
Kim Yu-Jin
Lee Seung-Hyung
Cheong Hee-Tae
Yang Boo-Keun
Park Choon-Keun
Hwang Bo-Yong
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Abstract
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This study investigated the effects of L-carnitine (LC) and nicotinic acid (NA) on sperm viability during liquid storage at 18℃ in miniature pigs. 10μM LC and 30 mM NA, combined LC and NA (LN) were treated in fresh semen for 3, 7, and 10 days. In results, sperm survival increased in NA- and LN-treated semen on 7 and 10 days (p<0.05), mitochondrial integrity of live sperm increased in LN-treated semen on 7 days (p<0.05), but not NA-treated semen. In addition, we examined the acrosome reaction of sperm in miniature pigs. LC and NA did not influence on acrosome reaction of boar sperm. In conclusion, LC and NA effectively maintained the viability and quality of sperm during long-term storage in miniature pigs, suggesting that the combined LN may be useful for improving the semen extender for long-term liquid storage in pigs.
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KEYWORD
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L-carnitine, Nicotinic acid, Sperm viability, Miniature pigs
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