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KMID : 1023620160400040033
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Reproductive and Developmental Biology
2016 Volume.40 No. 4 p.33 ~ p.37
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Effects of α-Linolenic Acid and Bovine Serum Albumin on Frozen-thawed Boar Sperm Quality during Cryopreservation
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Lee Won-Hee
Hwangbo Yong
Lee Sang-Hee
Cheong Hee-Tae
Yang Boo-Keun
Park Choon-Keun
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Abstract
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This study was conducted to evaluate effect of α-linolenic acid (ALA) and bovine serum albumin (BSA) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-
hand method and cryopreserved using freezing extender containing 3 ng/mL ALA and/or 20 μg/mL BSA. Cryopreserved
boar sperms were thawed in 37℃ water-bath for 45 sec to analysis. Viability, acrosome reaction, and mitochondrial
intact were analyzed using flow cytometry. In results, viability of frozen-thawed boar sperm was significantly higher in only ALA+BSA supplement group than control group (p<0.05), whereas there was no difference either in ALA or BSA supplement. However, acrosome reacted sperm in both of live and all sperm population were significantly decreased in all treatment groups than control (p<0.05). Interestingly, mitochondrial intact of boar sperm was enhanced in ALA and ALA+BSA groups compared with control (p<0.05). In this study, we showed that supplementation of ALA and BSA in freezing extender enhanced the sperm viability, mitochondrial intact and decrease acrosomal membrane damage. In conclusion, our findings suggest that quality of frozen-thawed sperm in mammalians could improve by using of ALA and BSA.
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KEYWORD
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Alpha-linolenic acid, Bovine serum albumin, Cryopreservation, Sperm characteristics, Pigs
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