|
KMID : 1023820150070060468
|
|
The Journal of Advanced Prosthodontics
2015 Volume.7 No. 6 p.468 ~ p.474
|
|
|
Partial denture metal framework may harbor potentially pathogenic bacteria
|
|
Cristiane Machado Mengatto
Leonardo Marchini
Luciano Angelo de Souza Bernardes
Sabrina Carvalho Gomes
Alecsandro Moura Silva
Celia Marisa Rizzatti-Barbosa
|
|
|
Abstract
|
|
|
PURPOSE : The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time.
MATERIALS AND METHODS : This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity).
RESULTS : A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2.
CONCLUSION : The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.
|
|
|
KEYWORD
|
|
|
Framework, Denture plaque, Microorganisms, 16S rDNA gene, Removable partial denture
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|