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KMID : 1024620020220030274
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Food Science of Animal Resources
2002 Volume.22 No. 3 p.274 ~ p.280
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Effect of -Casein, GMP and Sialic Acid on the Infection of MA-104 Cells by Korean Native Cattle Rotavirus and JBR
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Yu Jae-Hyeun
Kim Jong-Heon
Park Bum-Suk
Yu Dae-Hwan
Shin Won-Sun
Kim Se-Min
Ji Byoung-Ju
Song Jin-Ook
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Abstract
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This study was conducted to investigate inhibitory effects of K-casein, GMP and sialic acid addition on the infection of MA-104 cells by 597(Korean native cattle rotavirus) and JBR(Jeju island bovine rotavirus). MA-104 cells on incomplete Ml99 were infected with domestically separated 597 and ma activated by incubating at 37 for 6 days, and analyzed for the titer of rotavirus. K-casein, GMP and sialic acid added MA-104 culture infected by activated S97 and nan were incubated for Is hours and stained by the AEC stainning method. The number of infected cells were counted on microscope. The titer of S97 and JBR was 2.5107 and 2.0106 PFU/ml, respectively. The inhibition level against cell infection by 597 was 97.4% far 2000UH of K-casein and 97.44% for 2000UM of GMP. The inhibition level against cell infection by JBR was 99.52% for 2000M of -casein and 99.78% for 2000M of GMP. The inhibition level against cell infection by 597 and JBR was 3.85 and 3.63% for 2000M of sialic acid, respectively. The high inhibitory effects (over 97%) of K-casein and CMP against infection of U-1(14 cells with 597 and mR indicated great potentials for the use of K-casein and GMP in the treatment of calf or infant caused by rotavirus.
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KEYWORD
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-Casein, GMP, sialic acid, BRV S97, JBR, AEC staining, diarrhea
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