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KMID : 1024620030230030278
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Food Science of Animal Resources
2003 Volume.23 No. 3 p.278 ~ p.283
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Feasibility Study on the Use of Liposomes for Detecting Food-borne Pathogenic Bacteria
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Kim Myung-Hee
Kim Wang-June
Shin Weon-Sun
Shon Dong-Hwa
Cha Seong-Kwan
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Abstract
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Feasibility tests on using liposomes for detecting food-borne pathogenic bacteria were studied with E. coli 0157:H7 as a model analyte. lmmunoliposomes, whose surface was conjugated with anti-E. coli 0157:H7 IgG and which encapsulated the marker dye, sulforhodamine B, were used for the detection label. Among the feasibility tests, the first test was to use a test-strip on which antibodies to anti-E. coli O157:H7 IgG were immobilized. In this format, immunoliposomes that did not bind to E. coli O157:H7 in sample were captured and then exhibited a visible signal which was inversely related with the number of E. coli O157:H7 in sample. The second test was a direct liposome assay followed by immunomagnetic separation. In this format, immunoliposomes which were bound to E. coli O157:H7 were lysed with detergent and produced a signal which was proportionally related with the number of E. coli O157:H7 in sample. The results from both formats indicate that liposomes can be utilized as a detection label.
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KEYWORD
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liposome, test-strip, immunomagnetic bead, E. coli O157:H7, immunoassay, safety
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