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KMID : 1024620050250040494
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Food Science of Animal Resources
2005 Volume.25 No. 4 p.494 ~ p.499
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The Antioxidant Effect of Lactobacillus gasseri KACC 91155 Isolated from Korean Infant in Jurkat T Cells
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Jeong Seok-Keun
Kim Hyun-Soo
Ham Jun-Sang
Chae Hyun-Seok
Lee Jong-Moon
Ahn Chong-Nam
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Abstract
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In the present study, we investigate the protective effect of antioxidant strain Lactobacillus gasseri KACC 91155, isolated from Korean infant feces(Obstetrics & Gynecology, Suwon, Korea) on the oxidative stress damage on the Jurkat T cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA(malondialdehyde) production was measured Furthermore, cell viability was detected by the MTT assay, DNA damage was tested by the comet assay. Cell grown in medium with or without L gasseri lysate were treated with as an oxidative stimulus. From the result obtained, the supplementation of Jurkat T cells with L. gasseri lysate significantly decreased in MDA production (1,250 vs. 835 nmol/mg protein), and DNA damage(31.6 vs. 22.6 tail moment). Also L gasseri increase cell viability against oxidative damage. We concluded that the L. gasseri KACC 91155 showed a protective effect against oxidative stress.
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KEYWORD
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Lactobacillus gasseri, antioxidant, Jurkat T cell, DNA damage
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