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KMID : 1024620060260010113
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Food Science of Animal Resources
2006 Volume.26 No. 1 p.113 ~ p.120
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Effects of IGF-I Rich Fraction from Bovine Colostral Whey on Immune Activity of Mouse In Vitro
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Hwang Kyung-A
Yang Hee-Jin
Lee Soo-Won
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Abstract
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Insulin-like growth factor-I(IGF-I) rich fraction, which was obtained molecules ranged between 30 and 1 kDa, was fractionated by ultrafiltration from bovine colostral whey. IGF-I rich fraction was confirmed by SDS-PACE and western blotting and then the quantity of IGF-I was measured by sandwich ELISA. ICF-I concentration in IGF-I rich fraction was 10 ng/mg proteins. IGF-I rich fraction, standard IGF-I and colostral whey weie treated to murine peritoneal macrophages. And then we experimented that effect of immune activity on macrophage and splenocyte. As a result, in group treated with IGF-I rich fraction , production of interleukin-6 and nitric oxide were 9.85 ng and and production of phagocytosis, tumor necrosis factor- were 78.3, 34.5 and 6% compared to the control group. In splenocyte immune response, B cell and T cell proliferation and NK cell activity were 103, 126 and 22.2% in group treated with IGF-I rich fraction to compared to the control, respectively.
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KEYWORD
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IGF-I, macrophage, splenocyte, immune response
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