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KMID : 1024620080280010063
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Food Science of Animal Resources
2008 Volume.28 No. 1 p.63 ~ p.68
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Development of Rapid Somatic Cell Counting Method by Using Dye Adding NIR Spectroscopy
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Kim Kee-Sung
Noh Hae-Won
Lim Sang-Dong
Coi Chang-Hyun
Kim Yong-Joo
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Abstract
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To develop the somatic cell counting NIR Spectrum method within a range of 400-2500 nm, eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were tested at 0.01% in raw milk. The PLS (Partial Least Square) results are summarized as follows: Correlation coefficients of the calibration model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.78, 0.65, 0.63, 0.65, 0.98 and 0.99, respectively. The correlation coefficients of the prediction model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.49, 0.21, 0.36, 0.47, 0.95 and 0.98 respectively. Based on these results, amido black 10B was the best additive for the NIRS somatic cell count method.
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KEYWORD
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somatic cell count, NIR spectroscopy, raw milk, dye addition
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