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KMID : 1024620100300020328
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Food Science of Animal Resources
2010 Volume.30 No. 2 p.328 ~ p.335
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A Study on the Sensory Characteristic of Yogurt and Antimicrobial Activity of Lactobacillus plantarum LHC52 Isolated from Kimchi
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Lee Seung-Kyu
Han Gi-Sung
Jung Seok-Geun
Oh Mi-Hwa
Jang Ae-Ra
Kim Dong-Hun
Bae In-Hyu
Ham Jun-Sang
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Abstract
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The aim of our study was to develop a new starter culture for fermented milk. Polymerase chain reaction screening of 103
acid-producing isolates from Kimchi identified 72 Lactobacillus strains. The ability of the strains to inhibit the growth of the
food-borne human pathogens (Escherichia coli, Salmonella Enteritidis, Staphylococcus aureus) was measured, using a conventional paper disk method. Among the 72 strains, strain LHC52 displayed potent antagonistic activity. Use of 16S rDNA sequencing and the API 50CHL system identified the strain as Lactobacillus plantarum and it was designated L. plantarum LHC52. Biochemical analyses revealed especially high antibacterial activity against E. coli. Yogurt produced using L. plantarum LHC52 did not show different microbiological and physicochemical properties compared to conventionally-prepared yogurt, implicating L. plantarum LHC52 as a useful, potently antibacterial starter culture for yogurt preparation.
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KEYWORD
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Kimchi, Lactic acid bacteria, Lactobacillus plantarum, antibacterial activity, fermented milk
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