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KMID : 1024620100300060918
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Food Science of Animal Resources
2010 Volume.30 No. 6 p.918 ~ p.922
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Discrimination of Korean Cattle (Hanwoo) with Imported Beef from USA Based on the SNP Markers
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Shim Jung-Mi
Seo Dong-Won
Seo Seong-Won
Kim Jong-Joo
Min Dong-Myung
Kim Ik-Chul
Jeon Jin-Tae
Lee Jun-Heon
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Abstract
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Due to the large amount of beef imported from the USA to Korea, Korean consumers have become increasingly interested in the country of origin since it can affect market prices. Previously, Bos indicus and Bos taurus-specific markers were developed for the purpose of cattle breed identification, specifically discrimination of Australian beef. In this study, six SNP markers derived from Illumina 50K bovine SNP chip data were used for the discrimination between Korean cattle (Hanwoo) and imported beef from USA. PCR-RFLP genotyping methods were also developed, which indicates that these markers can be applied relatively easily compared to other markers. Taking into account a discrimination rate of 55% based on MC1R marker between Hanwoo and imported beef from USA, two additional markers, SNPs 23803 and 34776, were ideal and resulted in probability of identification of 0.942 and probability of misjudgment of 0.03. Therefore, the markers developed in this study can greatly contribute to the correct discrimination between beef from USA and Hanwoo beef.
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KEYWORD
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MC1R, Korean cattle (Hanwoo), SNP marker, PCR-RFLP, discrimination
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