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KMID : 1024620100300060923
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Food Science of Animal Resources
2010 Volume.30 No. 6 p.923 ~ p.929
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Production of Iron-Binding Peptides from Colostral Whey by Enzymatic Hydrolysis
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Kim Sang-Bum
Ku Min-Jung
Cho Won-Mo
Ki Kwang-Seok
Kim Hyeon-Shup
Nam Myoung-Soo
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Abstract
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Colostral whey prepared from colostrum (pooled from first six post-partum milkings) was heated for 10 min at 100℃ Heated colostral whey was incubated with 1% enzymes (protein equivalent basis) for 15, 30, 60, 90, and 120 min at 50℃. Papain, pepsin, trypsin, and alcalase produced different degrees of hydrolysis (DH), 10.66%, 12.42%, 10.83%, and 25.31%, respectively, at an incubation time of 120 min. The SDS-PAGE reveals that significant amounts of bovine serum albumin (BSA), β-lactoglobulin (β-LG), and α-lactalbumin (α-LA) survived papain digestion. In contrast, pepsin completely removed BSA but not β-LG present in heated colostral whey. Alcalase completely eliminated BSA, β-LG, and α-LA. This differential hydrolysis was confirmed by reversed-phase HPLC analysis. Using ion-exchange chromatography, fraction-1 (F-1) was obtained from alcalase hydrolysate at a NaCl gradient concentration of 0.25 M. Reversed-phase HPLC chromatograms of alcalase F-1 showed numerous small peaks, which probably indicate that a variety of new peptides were produced. Iron content of alcalase F-1 was 28.94 ppm, which was the highest among all enzyme fractions, whereas iron content of colostral whey was 36.56 ppm. Main amino acids contained in alcalase F-1 were Thr (15.45%), Glu (14.12%), and Ser (10.39%). Therefore, alcalase can be used to generate good iron-binding peptides in heated colostral whey, and the resulting iron-binding peptides could be suitable as a value-added food ingredient for food supplements.
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KEYWORD
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Colostral whey, hydrolysis, alcalase, iron-binding peptide
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