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KMID : 1024620110310050663
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Food Science of Animal Resources
2011 Volume.31 No. 5 p.663 ~ p.667
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Assessment of the Inhibitory Activity of Peptide Extracts from Hanwoo Musculus Longissimus on Angiotensin I-Converting Enzyme
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Seol Kuk-Hwan
Song Ji-Hye
Thirawong Prayad
Kim Hyoun-Wook
Jang Ae-Ra
Ham Jun-Sang
Oh Mi-Hwa
Kim Dong-Hun
Lee Moo-Ha
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Abstract
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This study was performed to measure the angiotensin I-converting enzyme (ACE) inhibitory activity of peptide extracts derived from the enzymatic proteolysis of Hanwoo Musculus longissimus (M. longissimus) during cold storage. Thermolysin (80 ppm, w/w) and protease type XIII (100 ppm, w/w) were injected separately or in combination for the enzymatic proteolysis of sarcoplasmic and myofibrillar proteins prior to storage at 5℃ (T1) or at -1℃ (T2) in a chilling room for 9 days. Beef injected with thermolysin (E2) and thermolysin+protease type XIII (E3) showed a significantly higher degree of hydrolysis at both storage temperatures (p<0.05). During the storage period, T1E2 at day 6 and T1E3 at day 9 showed the strongest ACE inhibitory activity with sarcoplasmic and myofibrillar protein proteolysates. Macromolecules greater than 10,000 Da were removed by ultra filtration, and the filtrates were separated into fractions using gel filtration. Five and three major fractions were collected from S-T1E2-6 and M-T1E3-9 extracts, respectively, and the 4th fraction of the S-T1E2-6 extracts showed the highest ACE inhibitory rate of 61.96±7.41%.
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KEYWORD
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angiotensin I-converting enzyme inhibitory activity, Hanwoo beef, thermolysin, protease type XIII
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