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KMID : 1024620110310060944
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Food Science of Animal Resources
2011 Volume.31 No. 6 p.944 ~ p.951
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Development of Analysis Method for Cholesterol in Infant Formula by Direct Saponification
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Kim Jin-Man
Park Jung-Min
Yoon Tae-Hyung
Leem Dong-gil
Yoon Chang-Yong
Jeong Ja-Young
Jeong In-Seek
Kwak Byung-Man
Ahn Jang-Hyuk
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Abstract
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An improved cholesterol analysis method was developed for powdered infant formula by gas chromatographic separation after liquid-liquid extraction and partition. In the official Korea Food Standard method for cholesterol analysis, the water phase and solvent phase were not well separated in the case of emulsified foods such as powdered infant formulas and baby foods. For the rapid and simple sample preparation method, an optimized direct saponification condition was established for heating temperature, heating time, and KOH concentration. From the results, the optimum conditions were as follows: heating temperature 90℃, heating time 60 min, and 16 M KOH 10 mL for a 2 g infant formula sample; improved separation condition for gas chromatography was as follows: the initial oven condition was 250℃ for 25 min, the oven temperature was increased to 290℃ by 10℃/min ratio, and finally the oven temperature remained at 290℃for 9 min. The developed method could be implemented for the study of cholesterol, providing the advantages of reduced inspection time and cost in emulsified foods such as infant formula.
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KEYWORD
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cholesterol, direct saponification, gas chromatography, analysis method, infant formula
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