|
KMID : 1024620120320010062
|
|
Food Science of Animal Resources
2012 Volume.32 No. 1 p.62 ~ p.67
|
|
|
Application of Probabilistic Model to Calculate Probabilities of Escherichia coli O157:H7 Growth on Polyethylene Cutting Board
|
|
Lee Joo-Yeon
Suk Hee-Jin
Lee Hee-Young
Lee Soo-Min
Yoon Yo-Han
|
|
|
Abstract
|
|
|
|
This study calculated kinetic parameters of Escherichia coli O157:H7 and developed a probabilistic model to estimate growth probabilities of E. coli O157:H7 on polyethylene cutting boards as a function of temperature and time. The surfaces of polyethylene coupons (3x5 cm) were inoculated with E. coli O157:H7 NCCP11142 at 4 Log CFU/㎠. The coupons were stored at 13 to $35^{\circ}C$ 수식 이미지 for 12 h, and cell counts of E. coli O157:H7 were enumerated on McConkey II with sorbitol agar every 2 h. Kinetic parameters (maximum specific growth rate, Log CFU/㎠/h; lag phase duration, h; lower asymptote, Log CFU/㎠; upper asymptote, Log CFU/㎠) were calculated with the modified Gompertz model. Of 56 combinations (temperatureχtime), the combinations that had ≥0.5 Log CFU/㎠ of bacterial growth were designated with the value of 1, and the combinations that had increases of <0.5 Log CFU/㎠ were given the value 0. These growth response data were fitted to the logistic regression to develop the model predicting probabilities of E. coli O157:H7 growth. Specific growth rate and growth data showed that E. coli O157:H7 cells were grown at 28-35℃, but there were no obvious growth of the pathogen below 25℃. Moreover, the developed probabilistic model showed acceptable performance to calculate growth probability of E. coli O157:H7. Therefore, the results should be useful in determining upper limits of working temperature and time, inhibiting E. coli O157:H7 growth on polyethylene cutting board.
|
|
|
KEYWORD
|
|
|
Escherichia coli O157:H7, probabilistic model, predictive model, cutting board
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|