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KMID : 1024620120320050652
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Food Science of Animal Resources
2012 Volume.32 No. 5 p.652 ~ p.658
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Effect of Sodium Caseinate Hydrolysates on Angiotensin-I Converting Enzyme Inhibition Activity
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Lee Keon-Bong
Shin Yong-Kook
Baick Seung-Chun
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Abstract
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This study was carried out to identify the ACE (Angiotensin converting enzyme) inhibitory activity of casein hydrolysates for development of anti-hypertensive hydrolysates. Sodium caseinate was treated with six kinds of commercial proteases such as Flavourzyme, Protamex, Neutrase 1.5, Alcalase, Protease M, and Protease S for 8 h individually, and was then treated with the enzyme combination for 4 h at 45℃. The hydrolysate which had the highest ACE inhibitory effect was then hydrolysed successively with three digestive enzymes: pepsin, trypsin, and α-chymotrypsin, at 37℃ for 4 h under conditions mimicking those of the gastrointestinal tract. UF (ultra filtration) treatment was applied to one of the secondary hydrolysates to determine ACE inhibitory activity. When sodium caseinate was hydrolysed by commercial proteases, the degree of hydrolysis (DH) showed 2.54 to 4.25% and after secondary hydrolysis, DH showed 4.30 to 5.22%. ACE inhibitory activity and IC_50 values decreased, and inhibition rates increased during hydrolysis. Protamex treatment showed the lowest IC_50 value (516㎍/mL) and Flavourzyme hydrolysate showed the highest IC_50value (866㎍/mL). As the first hydrolysate was treated with Flavourzyme, the ACE inhibitory activity increased. Neutrase hydrolysate had the highest activity with an IC_50 value (282㎍/mL). When Neutrase plus Flavourzyme treatment was hydrolyzed by digestive enzymes, the IC_50 value (597㎍/mL) was decreased statistically (p<0.05). As Neutrase plus Flavourzyme hydrolysate is treated by UF with MW cut-off 10,000, permeate showed 273㎍/mL of IC_50 value, showed no difference, but retentate which has over MW 10,000 showed statistically different IC_50 value, 635㎍/mL(p<0.05).
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KEYWORD
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angiotensin converting enzyme, sodium caseinate, hydrolysate, protease, digestive enzyme
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