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KMID : 1024620130330020198
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Food Science of Animal Resources
2013 Volume.33 No. 2 p.198 ~ p.204
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The Application of ParalluxTM System for Multi-Detection of (Fluoro)quinolone Class Antibiotics Residues in Raw Bovine Milk
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Park Hong-Je
Kim Gyung-Dong
Han Kyu-Ho
Lee Chi-Ho
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Abstract
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This study aimed to apply the Parallux system to detect (fluoro)quinone antibiotics residues in raw bovine milk. The immunogen enabled the generation of a specific antiserum with a titer of 1/40,000. The Parallax^{TM} kit using the antibody displayed IC_{50} value of 10 to 150 ppb for (fluoro)quinolone antibiotics. Parallax^{TM} kit was also sensitive for the detection of incurred (fluoro)quinolone at Korean Maximum Residual Levels in raw bovine milk as the result of dose response test. Cross reactivities of the antibody with the common (fluoro)quinolones were determined to be norfloxacin, 100%; enrofloxacin, 100%; ciprofloxacin, 100%; danofloxacin, 100%; nalidixic acid, 40%. Lower detection limit (LOD) values of the Parallax^{TM} kit in raw bovine milk were determined to be norfloxacin, 4 ppb; enrofloxacin, 5 ppb; danofloxacin, 5 ppb; ciprofloxacin, 5 ppb and nalidixic acid, 10 ppb. The Parallax^{TM} kit was run 8 times with five different concentrations of norfloxacin to determine the coefficient of variation (CV, %) of intra-assay, which was between 2.7% and 11.8%. To confirm the precision among kit batches for the inter-assay, five different batch kits were tested with 2 different concentration of norfloxacin. The CVs of the inter assay were 4.2% at 50 ppb, and 7.2% at 10 ppb norfloxacin, respectively.
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KEYWORD
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(fluoro)quinolone, Parallax^{TM} system, ELISA, immunoassay, cross activity
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