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KMID : 1024620150350050707
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Food Science of Animal Resources
2015 Volume.35 No. 5 p.707 ~ p.713
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Whitening Effect of Watersoluble Royal Jelly from South Korea
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Han Sang-Mi
Kim Jung-Min
Hong In-Phyo
Woo Soon-Ok
Kim Se-Gun
Jang Hye-Ri
Park Kwan-Kyu
Pak Sok-Cheon
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Abstract
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Royal jelly has been widely used as a health supplement worldwide. However, royal jelly has been implicated in allergic reactions, and we developed a water-soluble royal jelly (WSRJ) without the allergy inducing protein. In this study, we aimed to identify the anti-melanogenic efficacy of WSRJ. B16F1 melanoma cells were first treated with 10 nM α-melanocyte stimulating hormone (α-MSH) and then with various doses of WSRJ. In addition, we investigated the mRNA and protein expression of melanogenesis-related genes such as tyrosinase, tyrosinase related protein-1 (TRP-1) and TRP-2 by reverse transcription-polymerase chain reaction and western blotting. WSRJ directly inhibited tyrosinase and cellular tyrosinase activity, which decreased melanin synthesis in α-MSH stimulated B16F1 melanoma cells a level comparable to that observed with arbutin. WSRJ decreased the mRNA and protein expressions of tyrosinase, TRP-1, and TRP-2, which was comparable to that observed with arbutin. WSRJ has strong anti-melanogenic activity, which invoice direct inhibition of tyrosinase enzyme activity and suppression of expression of melanogenesis related genes. Results from this study suggests that WSRJ is a potential candidate for the treatment of skin pigmentation.
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KEYWORD
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royal jelly, water-soluble royal jelly, α, -MSH, tyrosinase, TRP-1, TRP-2
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