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KMID : 1024620190390010084
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Food Science of Animal Resources
2019 Volume.39 No. 1 p.84 ~ p.92
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Simple Evaluation of Listeria monocytogenes Pathogenesis Using Caenorhabditis elegans Animal Model
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Yang Kyoung-Hee
Yun Bo-Hyun
Choi Hye-Jin
Ryu Sang-Don
Lee Woong-Ji
Oh Mi-Hwa
Song Min-Ho
Kim Jong-Nam
Oh Sang-Nam
Kim Young-Hoon
Kim Young-Jun
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Abstract
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Listeria monocytogenes is a major cause of serious foodborne illness in the dairy foods. Although Caenorhabditis elegans model is well established as a virulence model of pathogenic bacteria, its application on L. monocytogenes is critically unclear. The objective of this study was to carry out an evaluation of L. monocytogenes toxicity using C. elegans nematode as a simple host model. We found that C. elegans nematodes have high susceptibility to L. monocytogenes infection, as a consequence of accumulation of bacteria in the worms' intestine. However, L. innocua, which is known to be non-toxic, is not accumulate in the intestine of worms and is not toxic similarly to Escherichia coli OP50 known as the normal feed source of C. elegans. Importantly, immune-associated genes of C. elegans were intensely upregulated more than 3.0-fold when they exposed to L. monocytogenes. In conclusion, we established that C. elegans is an effective model for studying the toxicity of L. monocytogenes and we anticipate that this system will result in the discovery of many potential anti-listeria agents for dairy foods.
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KEYWORD
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Listeria monocytogenes , Caenorhabditis elegans , toxicity , immune response
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