KMID : 1024620200400050746
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Food Science of Animal Resources 2020 Volume.40 No. 5 p.746 ~ p.757
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Genetic Analysis and Characterization of a Bacteriophage ¨ªCJ19 Active against Enterotoxigenic Escherichia coli
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Kim Gyeong-Hwuii
Kim Jae-Won Kim Jae-Gon Chae Jong-Pyo Lee Jin-Sun Yoon Sung-Sik
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Abstract
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Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ¨ªCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ¨ªCJ19 belongs to the family Myoviridae. One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4¡É and 55¡É, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ¨ªCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ¨ªCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ¨ªCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.
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KEYWORD
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animal husbandry, animal source food, bacteriophage, enterotoxigenic Escherichia coli, genome annotation
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