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KMID : 1034820050010020116
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Molecular & Cellular Toxicology
2005 Volume.1 No. 2 p.116 ~ p.123
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Effects of Hyperbaric Pressure on Cellular Morphology, Proliferation and Protein Expression of Jurkat Cell
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Oh Eun-ha
Oh Sang-Nam
Im Ho-Sub
Moon Joo-Hee
Hong Eun-Young
Kim Jin-Young
Lee Joo-Hyun
Kim Yang-Hee
Yang Min-Ho
Lim Yong-Chul
Park Sun-Young
Lee Eun-Il
Sul Dong-geun
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Abstract
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The application of high pressure on cellular morphology, proliferation and protein expression of Jurkat cells (human T lymphocyte cell line) has been extensively investigated. In the present study, we manufactured a novel pressure chamber that modulates 5% , temperature and pressure (up to 3 ATA). Jurkat cells was incubated 2 ATA pressure and analyzed cellular morphology and growth using an electron microscopy and MTT assay. The cells showed the morphological changes in the cell surface, which appeared to cause a severe damage in cell membrane. The growth rate of the cells under 2 ATA pressure decreased as cultured time got increased. Furthermore, a long term exposure of high pressure on Jurkat cells may act as one of the important cellular stresses that leads to inducing cell death. Cellular proteomes were separated by 2-dimensional electrophoresis with pH 3-10 ranges of IPG Dry strips. And many proteins showed significant up-and-down expressions with hyperbaric pressure. Out of all, 10 spots were identified significantly using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. We and found that 9 protein expressions were decreased and one protein, heat shock protein HSP 60, was increased in Jurkat cells under 2 ATA. Identified proteins were related to lipid metabolism and signal transduction.
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KEYWORD
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hyperbaric pressure, Jurkat cells, cell growth, 2-dimensional electrophoresis, HSP60
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