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KMID : 1034820050010040237
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Molecular & Cellular Toxicology
2005 Volume.1 No. 4 p.237 ~ p.247
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Biological Effects of Different Thin Layer Hydroxyapatite Coatings on Anodized Titanium
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Sohn Sung-Hwa
Jun Hye-Kyoung
Kim Chang-Su
Kim Ki-Nam
Ryu Yeon-Mi
Lee Seung-Ho
Kim Yu-Ri
Seo Sang-Hui
Kim Hye-Won
Shin Sang-Wan
Ryu Jae-Jun
Kim Meyoung-Kon
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Abstract
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Several features of the implant surface, such as roughness, topography, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of various thin layer hydroxyapatite (HA) coatings on anodized Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on A (100 nm HA coating on anodized surface), B (500-700 nm HA coating on anodized surface), C ( HA coating on anodized surface), and control (non HA coating on anodized surface) Ti. The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the four dental substrate types. MG63 cells cultured on A, C and control exhibited cell-matrix interactions. It was B surface showing cell-cell interaction. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.
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KEYWORD
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Titanium, hydroxyapatite coating, gene expression profiling, cDNA microarray, anodization
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