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KMID : 1034820050010040275
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Molecular & Cellular Toxicology
2005 Volume.1 No. 4 p.275 ~ p.280
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Toxicogenomics Study on Carbon Tetrachloride-induced Hepatotoxicity in Mice
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Jeong Sun-Young
Park Han-Jin
Lim Jung-Sun
Hwang Ji-Yoon
Cho Jae-Woo
Song Chang-Woo
Kim Yang-Seok
Lee Wan-Seon
Moon Jin-Hee
Han Sang-Seop
Yoon Seok-Joo
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Abstract
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Carbon tetrachloride () is well known hepatotoxicant. Its overdose cause severe centrilobular hepatic necrosis in human and experimental animals. We administered at low (0.2 mL/kg p.o.) and high (2 mL/kg p.o.) doses to mice. Mice were sacrificed at 24 h after administration. We evaluated liver toxicity by serum AST and ALT level and by microscopic observation. Using cDNA chip, we conducted gene expression analysis in liver. Mean serum activities of the hepatocellular leakage enzymes, ALT and AST, were significantly increased compare to control, respectively, in the low and high dose groups. H&E evaluation of stained liver sections revealed histopathological findings in mice. Moderate centrilobular hepatocellular necrosis was present in all treated mice. We found that gene expression pattern was very similar between low and high dose group. However, some stress related genes were differently expressed. These results could be a molecular signature for the degree of liver injury. Our data suggest that the degree of severity could be figure out by gene expression profiling.
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KEYWORD
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Carbon tetrachloride, hepatotoxicity, cDNA microarray, gene expression
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