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KMID : 1034820060020010035
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Molecular & Cellular Toxicology
2006 Volume.2 No. 1 p.35 ~ p.47
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Effects of Dioxin Exposed in Human by Using Radioactive cDNA Microarray
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Ryu Yeon-Mi
Kim Ki-Nam
Kim Hye-Won
Sohn Sung-Hwa
Lee Seung-Ho
Kim Yu-Ri
Seo Sang-Hui
Lee Seung-Min
Lee Eun-Il
Kim Meyoung-Kon
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Abstract
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2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD) are well known as the most toxic environmental compound in these days. Many researches are reported that dioxin produces multiple toxic effects, such as endocrine toxicity, reproductive toxicity, immunotoxicity and cancer. In this study, we carried to discover novel evidence for previously unknown gene expression patterns in human exposed to dioxin by using radioactive cDNA microarray. 548 workers who were divided into experimental and control groups according to their urinary Naphthol levels were enrolled in our study. Blood mRNA in human was isolated, and the gene expression profiles were analyzed by cDNA microarray. Gene expression analysis identified 52 genes which exhibited a significant change. In our study, most notably, genes involved in cell cycle, cell proliferation, signal transduction and apoptosis in human exposed to dioxin, such as CCND3, TSHR, and EFRN5, were up-regulated. In the current study, we observed gene expression of people that are exposed to dioxin using radioactive cDNA microarray. Through these results, we suggest when objects are exposed to toxic compounds, such as dioxin, the radioactive cDNA microarray may be using in sensitively detecting of cancerous change.
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KEYWORD
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Dioxin, Radioactive cDNA microarray, Gene expression
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