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KMID : 1034820060020010067
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Molecular & Cellular Toxicology
2006 Volume.2 No. 1 p.67 ~ p.73
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Detection of Serum Anti-Extracellular Protein Kinase a Autoantibodies as a Potential Tumor Marker
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Lee Seung-Ho
Kim Ki-Nam
Seo Sang-Hui
Sohn Sung-Hwa
Kim Yu-Ri
Kim Hye-Won
Choi Chul-Won
Kim Jun-Suk
Kim Meyoung-Kon
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Abstract
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In previous studies, it has been discovered that cancer cells not only overexpress regulatory subunit I (Rl)/protein kinase type I (PKA-I) but also secrete outside the cell an extracellular form of PKA (ECPKA) and that the ECPKA secretion detected in patients’ serum is obviously greater than that found in non-cancer patients or healthy subjects. We now found that ECPKA elicits the formation of serum autoantibodies that can serve as a cancer diagnostic and prognostic marker. To measure the presence of anti-ECPKA autoantibody in the human sera, basic methodology for ECPKA assay was established an enzyme-linked immunosorbent assay (ELISA). We obtained serum samples from 199 patients with different types of cancer, and also obtained 31 serum samples to compare with ECPKA concentrations from non-cancer patients and 119 normal volunteers. Compared with normal or non-cancer patient sera, we found that the frequency of anti-ECPKA autoantibody was significantly higher in cancer patients (88%) than in those without cancer (17%). Furthermore the presence of anti-ECPKA autoantibodies in the serum of cancer patients was highly correlated with the site of metastasis. The immunoassay developed for anti-ECPKA antibodies is highly sensitive and specific. Therefore, this discovery of an autoantibody-based cancer diagnostic may have serious clinical application and may become an important advance over current technology.
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KEYWORD
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Extracellular protein kinase A (ECPKA), Tumor marker, Diagnosis
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