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KMID : 1034820060020030159
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Molecular & Cellular Toxicology
2006 Volume.2 No. 3 p.159 ~ p.165
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Amygdalin Modulates Cell Cycle Regulator Genes in Human Chronic Myeloid Leukemia Cells
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Park Hae-Jeong
Baik Haing-Woon
Lee Seong-Kyu
Yoon Seo-Hyun
Zheng Long-Tai
Yim Sung-Vin
Hong Seon-Pyo
Chung Joo-Ho
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Abstract
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To determine the anticancer effect of D-amygdalin (D-mandelinitrole--D-gentiobioside) in human chronic myeloid leukemia cells K562, we profiled the gene expression between amygdalin treatment and control groups. Through 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of D-amygdalin was at the concentration of 5 mg/mL for 24 h. We performed cDNA microarray analysis and compared the gene expression profiles between D-amygdalin (5 mg/mL, 24 h) treatment and control groups. Among the genes changed by D-amygdalin, we paid attention to cell cycle-related genes, and particularly cell cycle regulator genes; because arrest of cell cycle processing was ideal tactic in remedy for cancer. In our data, expressions of cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), ataxia telangiectasia mutated (includes complementation groups A, C, and D) (ATM), cyclin-dependent kinase inhibitor 1C (p57, Kip2) (CDKN1C), and CHK1 checkpoint homolog (CHEK1, formally known as CHK1) were increased, while expressions of cyclin-dependent kinase 2 (CDK2), cell division cycle 25A (CDC25A), and cyclin E1 (CCNE1) were decreased. The pattern of these gene expressions were confirmed through RT-PCR. Our results showed that D-amygdalin might control cell cycle regulator genes and arrest S phase of cell cycle in K562 cells as the useful anticancer drug.
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KEYWORD
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Chronic myeloid leukemia, D-Amygdalin, cDNA microarray, Cell cycle regulator genes
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