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KMID : 1034820070030010036
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Molecular & Cellular Toxicology
2007 Volume.3 No. 1 p.36 ~ p.45
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Gene Expression of Osteosarcoma Cells on Various Coated Titanium Materials
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Sohn Sung-Hwa
Lee Jae-Bun
Kim Ki-Nam
Kim In-Kyoung
Lee Seung-Ho
Kim Hye-Won
Seo Sang-Hui
Kim Yu-Ri
Shin Sang-Wan
Ryu Jae-Jun
Kim Meyoung-Kon
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Abstract
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Several features of the implant surface, such as topography, roughness, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of different-coatings on Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on HA (Hydroxyapatite coating on Titanium), Ano (HA coating on anodized surface Titanium), Zr (zirconium-coating on Titanium), and control (non-coating on Titanium). The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the three dental substrate types. MG63 cells cultured on HA, Ano, Zr, and control exhibited cell-matrix interactions. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.
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KEYWORD
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Titanium, HA coating, Gene expression profiling, cDNA microarray
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