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KMID : 1034820070030020085
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Molecular & Cellular Toxicology
2007 Volume.3 No. 2 p.85 ~ p.89
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Efficient Gene Delivery through the Human Transferrin Receptor of Fibroblast-like Synoviocytes Stimulated with bFGF: a Potential Target Receptor for Gene Transduction in Rheumatoid Arthritis
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Kim Hak-Jae
Joung In-Sil
Nah Seong-Su
Lee Kyu-Hoon
Kim Kwon Yun-Hee
Chung Joo-Ho
Hong Seung-Jae
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Abstract
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Efficient gene delivery to specific tissues, such as inflammatory and cancerous tissues, is currently a major concern in disease treatment. The human transferrin receptor (hTR) has been detected in the synovium and fibroblast-like synoviocytes (FLS), which raises the possibility that expression of hTR is associated with the pathogenesis of rheumatoid arthritis (RA). To investigate whether the hTR is a useful target for gene transduction into the FLS of RA patients, recombinant adenoviruses with wildtype fiber (AdLac) and transferrin peptide-tagged fiber (Tf-AdLac) were used. The hTR expression level in FLS was notably increased by basic fibroblast growth factor (bFGF). Gene transduction to FLS was significantly higher by the hTR-targeted adenovirus than by the AdLac adenovirus, and treatment of the FLS with bFGF resulted in increased gene transduction by Tf-AdLac. Taken together, these data support Tf-AdLac as a new strategy for gene transduction in the treatment of RA patients.
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KEYWORD
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Fibroblast-like synoviocyte, Rheumatoid arthritis, Basic fibroblast growth factor, Human transferrin receptor, Recombinant adenovirus
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